Comparison of commercial DNA preparation kits for the detection of Brucellae in tissue using quantitative real-time PCR

Table 1 Mean concentration of Brucella melitensis DNA obtained with five purification kits from tissue samples of two dromedaries.

	QIAGEN		Peqlab		Macherey-Nagel		MoBio		Roche
fg/μl	mean	SD	mean	SD	mean	SD	mean	SD	mean	SD
D1452/06
Liver	8915	15150	186	17	76	9	neg		207	26
Spleen	10	2	5	2	101	21	neg*		402	20
Lung	225	66	72	11	216	55	71	23	2252	89
Right kidney	1377	19	73	27	315	24	101	19	757	53
Prescapular Ln	415	65	111	13	41	11	123	20	1338	355
Intestine	223	56	90	8	179	29	neg*		382	54
D1469/06
Spleen	484	426	57	4	38	7	neg*		67	67
Right kidney	235	23	10	8	14	3	3	3	111	29
Spinal Cord	289	64	11	1	126	33	neg*		14*	12
Pancreas	229	57	61	10	179	34	75	15	88*	14
Aorta lymph node	208	42	4	2	238	41	neg		112	11
Mammary Ln	355	299	140	26	297	12	465	60	112	18

(Ln, lymph node; SD, standard deviation; neg, negative Brucella PCR assay; *, negative inhibition control).

ISSN: 1471-2334