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Table 2 Results of the MTCD-MPCR with Bangladesh clinical isolates.

From: Identification of Mycobacterium tuberculosis clinical isolates in Bangladesh by a species distinguishable multiplex PCR

Species Interpretation

Banding pattern a

Number of isolates

%

 

cfp32

RD9

RD12

  

M. tuberculosis

+

+

+

347

99.1

M. bovis/M. caprae

+

-

-

0

0

"M. canettii"

+

+

-

1 c

0.3

Other MTC b

+

-

+

0

0

Non MTC

-

-

-

0

0

Irregular

-

+

+

2 d

0.6

Total

   

350

100

  1. aBanding patterns are shown as amplification results of the MTCD-MPCR in order of cfp32, RD9 and RD12: +, Amplification positive; -, negative.
  2. bOther MTC includes following species: M. africanum, M. microti, M. pinnipedii, dassie bacillus and oryx bacillus. The latter two are minor strains belonging to MTC [2].
  3. cConfirmed to be M. tuberculosis by hsp65 and gyrB sequencing.
  4. dConfirmed to be M. tuberculosis by gyrB sequencing.