Figure 3
ROC curve to determine optimal cutoff value by real-time PCR. ROC curve graphing sensitivity versus (1-specificity) for the prediction of determination of active CMV infection using pp65 antigenemia (1 positive pp65 cells/3 × 105 PML) as the reference standard for establishing the optimal cutoff level for real-time PCR. The optimal cutoff value for real-time PCR in peripheral blood leukocytes was 418.4 copies/104 PBL (sensitivity 71.4%; specificity 89.7%)