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Table 1 Comparison of virus isolation with real-time RT-PCR in hMPV detection

From: Comparison of virus isolation using the Vero E6 cell line with real-time RT-PCR assay for the detection of human metapneumovirus

Days of specimen collection after onset of illness

No. of specimens

No. of specimens with the following resultsa:

Sensitivity

Specificity

  

P+,C+

P+,C-

P-,C+

P-,C-

(%)

(%)

1

48

13

2

0

33

86.7

100

2

77

16

5

0

56

76.2

100

3

38

7

1

0

30

87.5

100

4

25

3

3

1

18

50

94.7

> 4

36

3

9

0

24

25

100

1-7

224

42

20

1

161

67.7

99.4

  1. aAbbreviations; P, real-time RT-PCR; C, cell culture