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Table 1 Primer sequences used in PCR for amplifying porB1A and porB1B genes

From: Predominant porB1A and porB1B genotypes and correlation of gene mutations with drug resistance in Neisseria gonorrhoeae isolates in Eastern China

Primers

Sequences (5' to 3')*

Products and sizes

porB1A/1B

F: ATGAAAAAATCCCTGATTGCC

981 bp for entire porB1A gene, and 1044 bp for entire porB1B gene

 

R: TTAGAATTTGTGGCGCAGAAC

 

porB1A/1B-D

F1: GCCATTTGGCAGTTGGAACA

520 bp for partial sequence of porB1A gene, and 201 bp and 583 bp for partial sequence of porB1B gene

 

F2: GATACGGCGAAGGCACTAAA

 
 

R: CTTCGGTTTGAGAGTTGTGC

 
  1. * F: forward primer, R: reverse primer