Table 3 Optimised conditions and PCR dynamics

Primer T_M (°C)

58.0

58.0

58.0

[Primer]

200 nM, both primers

200 nM, both primers

200 nM, both primers

[Probe]

100 nM

100 nM

100 nM

Slope

0.96842 ± 0.02787

0.97243 ± 0.03028

0.99701 ± 0.02886

Intercept

0.27100 ± 0.20289

0.21521 ± 0.19569

0.02605 ± 0.19293

Linearity (R2)

0.99424

0.99230

0.99334

SD of Residuals

0.21584

0.27500

0.26213

Efficiency (%)

99.42

100.4

99.26

Linear Range

4.85E+03 to 6.34E+10

1.52E+02 to 1.95E+10

1.38E+02 to 3.44E+10

LOD

4.85E+03 ± 3.02E+03

5.61E+02 ± 5.46E+02

2.59E+02 ± 2.48E+02

LOQ

4.85E+03 ± 3.02E+03

3.01E+02 ± 2.43E+02

1.38E+02 ± 1.17E+02