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Table 3 PCR/Electron spray ionization-time-of-flight-mass spectrometry (PCR/ESI-TOF-MS) versus traditional clinical microbiology (TCM) for detection of most frequently recovered potentially clinically relevant pathogens, plus coagulase negative staphylococci (CNS), contaminating healthcare workers and personal protective equipment

From: Comparison of PCR/Electron spray Ionization-Time-of-Flight-Mass Spectrometry versus Traditional Clinical Microbiology for active surveillance of organisms contaminating high-use surfaces in a burn intensive care unit, an orthopedic ward and healthcare workers

 

PCR/ESI-TOF-MS (n = 77)

TCM (n = 23)

Organism

Hands pre-care

Gloves

Gowns

Hands post-care

Hands pre-care

Gloves

Gowns

Hands post -care

CNS

12

6

8

12

4

4

3

3

Staphylococcus aureus

 

3

3

1

 

3

1

1

Streptococci*

9

4

3

1

 

1

  

Acinetobacter spp.**

 

3

1

  

1

  

Enterobacter spp.***

 

1

   

2

  

Escherichia coli

 

1

1

     

Klebsiella pneumoniae

1

3

1

1

    

Pseudomonas spp.****

1

  

1

    

Candida spp.*****

 

2

      
  1. *TCM: 1 Group D Streptococcus; PCR/ESI-TOF-MS: 7 S. agalactiae, 10 viridans group streptococci.
  2. **TCM: 1 A. baumannii; PCR/EIS-MS: 2 A. baumannii, 2 A. calcoaceticus.
  3. ***TCM: 2 Enterobacter aerogenes; PCR/ESI-TOF-MS: 1 E. aerogenes.
  4. ****PCR/ESI-TOF-MS: 1 Pseudomonas fluorescens, 1 Pseudomonas mendocina.
  5. *****PCR/ESI-TOF-MS: 1 C. albicans, 1 Candida glabrata.