Figure 2From: Quantitative discrimination of Aggregatibacter actinomycetemcomitans highly leukotoxic JP2 clone from non-JP2 clones in diagnosis of aggressive periodontitis Designation of the oligonucleotide primers and probes specific to the JP2 clone (above) and non-JP2 strains (below). For the JP2-specific amplification, the sense primer and hydrolysis probe are complementary to orfX' (for JP2 clone) and orfX (for non-JP2 strains). The antisense primer was designed to be complementary to the border of the 3' end of orfX' for the specific amplification of JP2 clones. For non-JP2-specific selection, the sense and antisense primers and TaqMan probe were designed to be complementary to the orfX ∆530 regionBack to article page