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Table 1 Oligonucleotide primers and hydrolysis probes used in this study

From: Quantitative discrimination of Aggregatibacter actinomycetemcomitans highly leukotoxic JP2 clone from non-JP2 clones in diagnosis of aggressive periodontitis

Primers or Probes

Sequence

Gene

Size

Primer

   

JP2-F3

5’-TCT ATG AAT ACT GGA AAC TTG TTC AGA AT-3’

orfX’

151 bpd

JP2-R2

5’-GAA TAA GAT AAC CAA ACC ACA ATA TCC-3’

orfX’

Non-JP2F

5’-CGC AAG TGC CAT AGT TAT CCA CT-3’

orfX (∆530 region)

145 bpe

Non-JP2R

5’-TCG TCT GCG TAA TAA GCA AGA GAG-3’

orfX (∆530 region)

Aa orfX3’F1

5’-AAA TCG TTG GCA TTC TCG -3’

orfX

723 bpe

Aa lktC5’R1

5’-CAA AGG AGA ATT TGC CCA -3’

lktC

Aa orfX3’F2

5’-CCG TTT TAT TCA GTT CCC -3’

orfX

809 bpe

Aa lktC5’R2

5’-TGC CCA TAA CCA AGC CAC -3’

lktC

Hydrolysis probesa, b, c

   

JP2

5’-FAM-ACA AAT CGT TGG CAT TCT CGG CGA A-TAMRA-3’

orfX’

 

Non-JP2

5’-FAM-ATA TTG TAG ACA TCG CCC-MGB-3’

orfX (∆530 region)

 
  1. aFAM, 6-carboxyfluorescein.
  2. bTAMRA, 6-carboxytetramethylrhodamine.
  3. cMGB, minor groove binder.
  4. dcalculated in JP2 strain.
  5. ecalculated in Y4 strain.