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Table 2 Primer specificity for the Aggregatibacter actinomycetemcomitans strains

From: Quantitative discrimination of Aggregatibacter actinomycetemcomitans highly leukotoxic JP2 clone from non-JP2 clones in diagnosis of aggressive periodontitis

Strain

Serotype

JP2/non-JP2

Amplification with primer pair:a

Primer pairs for Δ530 locus analysisb (for non-JP2)

Source or reference

   

JP2F3-JP2R2

NonJP2F- NonJP2R

  

ATCC 29523

a

non-JP2

-

+

1

ATCCc

SUNYaB 75 (ATCC 43717)

a

non-JP2

-

+

1

SUNYaBd

TN-1

a

non-JP2

-

+

2

Nishiharae

Y4 (ATCC 43718)

b

non-JP2

-

+

1

Socranskyf

JP2 (HK921)

b

JP2

+

-

-

Kiliang

HK 1199

b

JP2

+

-

-

Kilian

HK 1519

b

JP2

+

-

-

Kilian

HK 1611

b

JP2

+

-

-

Kilian

HK 1612

b

JP2

+

-

-

Kilian

HK 1651

b

JP2

+

-

-

Kilian

HK 1709

b

non-JP2

-

+

1

Kilian

ATCC 29522

b

non-JP2

-

+

2

ATCC

ATCC 29524

b

non-JP2

-

+

1

ATCC

HK 916

c

non-JP2

-

+

1

Kilian

SUNYaB 67 (ATCC 43719)

c

non-JP2

-

+

1

SUNYaB

NCTC 9709

c

non-JP2

-

+

2

NCTCh

NCTC 9710

c

non-JP2

-

+

none

NCTC

3381

d

non-JP2

-

+

2

Asikaineni

IDH 781

d

non-JP2

-

+

2

Asikainen

OMZ 534

e

non-JP2

-

+

2

Gmürj

OMZ 541

e

non-JP2

-

+

2

Gmür

OMZ 546

e

non-JP2

-

+

2

Gmür

NUM 5005

f

non-JP2

-

+

2

Takadak

NUM 4039

g

non-JP2

-

+

2

Takada

  1. a +, positive amplification with a band of the expected size; -, no amplification observed.
  2. b1, Aa orfX3’F1 and Aa lktC5’R1; 2, Aa orfX3’F2 and Aa lktC5’R2 (Table 1); none, not 1 or 2s.
  3. cATCC, American Type Culture Collection, Rockville, Md.
  4. dSUNYaB, State University of New York at Buffalo, NY.
  5. eNishihara, T. Nishihara, Kyushu Dental College, Kitakyushu, Japan.
  6. fSocransky, S. Socransky, Forsyth Dental Center, Mass.
  7. gKilian, M. Kilian, University of Aarhus, Aarhus, Denmark.
  8. hNCTC, National Collection of Type Cultures, London, England.
  9. iAsikainen, S. Asikainen, University of Helsinki, Helsinki, Finland.
  10. jGmür, R. Gmür, University of Zurich, Zurich, Switzerland.
  11. kTakada, K. Takada, Nihon University School of Dentisry at Matsudo, Chiba, Japan.