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Table 4 Comparison of the LAMP assay with nested PCR assay for the detection of the spotted fever rickettsiae group in clinical samples a

From: Rapid, simple, and sensitive detection of the ompB gene of spotted fever group rickettsiae by loop-mediated isothermal amplification

No. of cases

Diagnosis type

LAMP

Nested PCR

Real-time PCR

1

gltA gene amplified

Pos.

Neg.

Pos.

2

gltA gene amplified

Pos.

Neg.

Pos.

3

4-fold IgG titer increase

Pos.

Neg.

Neg.

4

Culture confirmed and gltA gene amplified

Pos.

Neg.

Pos.

5

4-fold IgG titer increase

Pos.

Neg.

Neg.

6

4-fold IgG titer increase and gltA gene amplified

Pos.

Neg.

Pos.

7

4-fold IgG titer increase and gltA gene amplified

Pos.

Neg.

Pos.

8

Suspected case

Neg.

Neg.

Pos.

9

Culture confirmed

Neg.

Neg.

Neg.

10

4-fold IgG titer increase

Neg.

Neg.

Neg.

11

4-fold IgG titer increase and gltA gene amplified

Pos.

Neg.

Pos.

  1. a All assays were performed in triplicate.