SNPs in the rickettsial
gene allowing species differentiation by real-time pyrosequencing. Used primers are marked by boxes. Note that Rick_pyro rev and Rick_pyro seq consist of complementary nucleotides and thus the lagging strand is sequenced. Detected SNPs are indicated by numbers. The displayed alignment is on GenBank accession numbers DQ910785 (R. helvetica), GQ925820 (R. monacensis), DQ459393 (R. massiliae), U33922 and GQ255903 (R. felis).