Expression of M. tuberculosis-induced suppressor of cytokine signaling (SOCS) 1, SOCS3, FoxP3 and secretion of IL-6 associates with differing clinical severity of tuberculosis

Table 2 Increased TNFα, IL-6 and IL-10 with down-regulated IFN-γ in tuberculosis along with disease spectrum

	Group	Un-stimulated	P value	M. tuberculosisstimulated	P value	Δ MTB	P value
	Mean ± SD	Mean ± SD		Mean ± SD		Mean ± SD
IFN-γ	EC	0.09 ± 0.3	NS	73.61 ± 95.8	0.281	78.786 ± 97.1	NS
	PTB	0 ± 0		18.889 ± 45.3		18.621 ± 45.4
	ETB	0.83 ± 3.9		56.70 ±173.8		57.522 ± 176.4
TNFα	EC	108.20 ± 134.7	NS	2541.29 ± 2877.2	0.573	2605.19 ± 2855.91	NS
	PTB	535.81 ± 1082.35		2860.30 ± 3076.9		2378.4 ± 3146.84
	ETB	340.02 ± 358.92		2056.48 ± 2005.8		1696.79 ± 2045.4
IL-2	EC	0.109 ± 0.4	NS	24.15 ± 53.9	0.249	24.045 ± 53.92	NS
	PTB	0.148 ± 0.51		23.18 ± 99.28		21.507 ± 97.6
	ETB	0.098 ± 0.404		36.87 ± 143.21		31.84 ± 134.92
IL-4	EC	0 ± 0	NS	0 ± 0	0.762	0 ± 0	NS
	PTB	0.083 ± 0.42		0.039 ± 0.2		0.37 ± 0.19
	ETB	0.375 ± 0.212		0.049 ± 0.288		0.049 ± 0.29
IL-6	EC	651.13 ± 1018.2	0.054	10804.35 ± 1301.5	0.215	11041.6 ± 9963.4	0.02
	PTB	6968.3 ± 8592.02*		11782.32 ± 9677.6		7125.8 ± 9578.9
	ETB	4677.9 ±5490.6		6581.54 ± 4757.47		2642.01 ± 3864.3*
IL-10	EC	0.29 ± 1.1	0.011	84.78 ± 168.3	0.874	90.54 ± 171.94	NS
	PTB	55.75 ± 234.4*		76.285 ± 217.01		42.758 ± 106.81
	ETB	51.33 ±169.17*		47.85 ± 91.7		24.76 ± 73.81
IL-17	EC	0 ± 0	NS	0 ± 0	1	0 ± 0	NS
	PTB	0.075 ± 0.38		0 ± 0		0 ± 0
	ETB	0 ± 0		0 ± 0		0 ± 0

EC, endemic control (n = 15); PTB, pulmonary TB (n = 33); ETB, extra-pulmonary TB (n = 33).
IQR, interquartile range between 25th and 75th percentile.
* Denotes significant difference (p ≤ 0.05) as compared with EC using Mann–Whitney U non-parametric test.
P value calculated using Kruskal Wallis test. NS denotes non-significant P value (p > 0.05).
Δ is calculated by substracting M. tuberculosis stimulated cytokine levels from background levels from un-stimulated cells.

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