Table 2 Brucella DNA amplification using B4/B5 and species-specific multiplex PCR
From: Two-stage PCR assay for detection of human brucellosis in endemic areas
(AFI) Samples locality | Brucellagenus B4/B5 conventional PCR | Brucellaspecies-specific multiplex | |||
|---|---|---|---|---|---|
(Western region) (number) | -ve amplification | +ve amplification | Double product | Single product | Single product |
Non- Brucellapatients | Brucellapatients | B. abortus & B. melitensis | B. abortus | B. melitensis | |
(Northern region) (61) | 11 | 50 | 41 (82%) | 5 (10%) | 4 (8%) |
(Central region) (40) | 40 | ------- | Not applicable | ||