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Table 1 Real-time PCR primers and probes[1618]

From: Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis

Oligonuclotide

Sequence

Final conc. (nM)

ctrA forward

5′-TGTGTTCCGCTATACGCCATT-3′

300

ctrA reverse

5′-GCCATATTCACACGATATACC-3′

900

ctrA probe

5′-FAM-AACCTTGAGCAA″T″CCATTTATCCTGACGTTCT-3′-SpC6*

100

bexA forward

5′-TGCGGTAGTGTTAGAAAATGGTATTATG-3′

600

bexA reverse

5′-GGACAAACATCACAAGCGGTTA-3′

600

bexA probe

5′-FAM-ACAAAGCGTATCAA″T″ACTACAACGAGACGCAAAAA-3′-SpC6*

100

lytA forward

5′-ACGCAATCTAGCAGATGAAGCA-3′

200

lytA reverse

5′-TCGTGCGTTTTAATTCCAGCT

200

lytA probe

5′-FAM-TGCCGAAAACGCTTGATACAGGGAG-3′-BHQ1

200

  1. * The quencher BHQ-1 is attached to the internal thymine residue “T”.