Cross-reactivities between human IgMs and the four serotypes of dengue virus as probed with artificial homodimers of domain-III from the envelope proteins

Table 4 Capacity of the (H6-ED3-PhoA) ₂ reagents to discriminate between DENV-infected and DENV-uninfected serums in a MAC-ELISA

(+) Serums	R1	R2	R3	R4
DENV1	0.94 ± 0.05	0.83 ± 0.06	0.64 ± 0.08	0.59 ± 0.08
DENV2	0.90 ± 0.03	0.88 ± 0.05	0.57 ± 0.06	0.42 ± 0.07
DENV3	0.82 ± 0.06	0.75 ± 0.07	0.77 ± 0.07	0.18 ± 0.08
DENV4	0.73 ± 0.05	0.55 ± 0.06	0.45 ± 0.06	0.61 ± 0.07
DENV1-4	0.83 ± 0.03	0.73 ± 0.04	0.59 ± 0.04	0.47 ± 0.04

R1 to R4, (H6-ED3-PhoA)₂ dimers of serotypes DENV1 to DENV4, respectively. The first column gives the serotype of the infecting DENV for the (+) serums. DENV1-4, the serotype of the infecting virus could be any one of DENV1 to DENV4. The DENV-uninfected serums were always taken as (−) serums. Each entry gives the accuracy and associated standard error of a MAC-ELISA that used the R_i reagent (i = 1, …, 4) as an antigen and was evaluated on the (+) and (−) serums.

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