Table 1 PCR assays selected for screening reference isolates
From: Detection of group a streptococcal pharyngitis by quantitative PCR
Target | Primer and probe sequences (5’-3’)* | Product size (nt) | Reference |
|---|---|---|---|
speB | 1F: GGTTCTGCAGGTAGCTCTCG | 346 | [17] |
1R: TGCCTACAACAGCACTTTGG | |||
2F: CTAAACCCTTCAGCTCTTGGTACTG | 77 | This study | |
2R: TTGATGCCTACAACAGCACTTTG | |||
probe: Cy3-CGGCGCAGGCGGCTTCAAC-BHQ2 | |||
parE | 1F: CAACAGATGCTACGGGATTGCAC | 139 | [18] |
1R: GTCAGTGTGGCAGATAGCGGACG | |||
spy1258 | 1F: AAAGACCGCCTTAACCACCT | 450 | [19] |
1R: TGGCAAGGTAAACTTCTAAAGCA | |||
2F: ACCTCAAATTTCCGCAACTC | 141 | This study | |
2R: TGCTCTCAATACTGGCAAGG | |||
probe: Cy3-TGGTTTCCAAGACATTGTGACCAATCA-BHQ2 | |||
spy1857 | 1F: CCTGCACCTGACATTTCAAC | 155 | This study |
1R: GAAGGTATTGAAGGCCGTGT |