Envelope protein gene based molecular characterization of Japanese encephalitis virus clinical isolates from West Bengal, India: a comparative approach with respect to SA14-14-2 live attenuated vaccine strain

Table 3 Comparison of E protein based amino acid substitutions identified in WB isolates with respect to wild type SA14-14-2 vaccine stain of JEV

Sl. no.	Identified E protein domains	Positions wise amino acid substitutions	Types of amino acid substitutions^a	Wild type vaccine strain	WB Isolates
Sl. no.	Identified E protein domains	Positions wise amino acid substitutions	Types of amino acid substitutions^a	SA14-14-2	IND/12/WB/JEV50	IND/11/WB/JEV47	IND/11/WB/JEV46	IND/11/WB/JEV45
1	Domain I	N2H	NC	N	H	H	H	H
2	Domain I	N8H	NC	N	.	H	.	.
3	Domain I	K138E	NC	K	E	E	E	E
4	Domain I	Q158P	C	Q	P	.	.	.
5	Domain I	V176I	C	V	I	I	I	I
6	Domain I	A177T	NC	A	T	T	T	T
7	Domain II	S64T	C	S	.	T	.	.
8	Domain II	N103K	NC	N	.	.	.	K
9	Domain II	F107L	NC	F	L	L	L	L
10	Domain II	S123R	NC	S	.	R	.	.
11	Domain II	A222S	NC	A	.	.	.	S
12	Domain II	G244E	NC	G	E	E	E	E
13	Domain II	G261S	NC	G	S	S	S	S
14	Domain II	H264Q	NC	H	.	Q	Q	Q
15	Domain II	M279K	NC	M	K	K	K	K
16	Domain III	E306G	NC	E	.	G	G	.
17	Domain III	A311R	NC	A	.	.	R	.
18	Domain III	V315A	C	V	.	A	A	A
19	Domain III	S327Q/T	C	S	.	Q	T	T
20	Domain III	S329T	C	S	.	T	T	.
21	Domain III	S331K	NC	S	.	K	.	.
22	Domain III	A366S	NC	A	.	S	S	.
23	Domain III	V372L	C	V	.	.	.	L
24	Domain III	M374I	C	M	.	.	.	I
25	Domain III	G388E	NC	G	.	E	.	K
26	Domain III	D389E	C	D	.	.	E	.
27	Domain III	W396R	NC	W	.	.	.	R
28	Stem	R416K	C	R	K	K	.	.
29	Stem	G432R	NC	G	.	R	.	.
30	Stem	R439K	C	R	.	K	K	K
31	Transmembrane	L463R	NC	L	.	.	R	.
32	Transmembrane	L467E	NC	L	.	E	.	.
33	Transmembrane	A481D	NC	A	.	D	.	.

Different amino acid substitutional positions were categorized into domains based on earlier reports [32, 33].
Identical amino acids in WB isolates with respect to wild type SA14-14-2 vaccine stain of JEV were indicated by “.”.
^aAmino acid substitutions were designated as C for conservative and NC for non-conservative.

ISSN: 1471-2334