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Table 2 Summary of dengue detection in serum samples from clinically dengue-suspected patients in UMMC using RT-LAMP, qRT-PCR, IgM and IgG ELISA

From: Detection of dengue viruses using reverse transcription-loop-mediated isothermal amplification

  

IgM ELISA

IgG ELISAb

  

Positive

Negative

Positive

Negative

Not-tested

Assays

Resultsc

n (%)

n (%)

n (%)

n (%)

n (%)

RT-LAMP

Positive

23 (30.7)

52 (69.3)

5 (6.7)

40 (53.3)

30 (40.0)

 

(n = 75d)

     
 

Negative

93 (40.4)

137 (59.6)

7f (3.1)

130 (56.5)

93 (40.4)

 

(n = 230)

     

qRT-PCR

Positive

25a (31.3)

55a (68.7)

5 (6.3)

43 (53.7)

32 (40.0)

 

(n = 80e)

     
 

Negative

91a (40.4)

134 (59.6)

7f (3.1)

127 (56.5)

91 (40.4)

 

(n = 225)

     
  1. aAcute DENV infection was confirmed in 171 samples.
  2. bDengue IgG ELISA was only performed on 182 (out of 189) dengue IgM negative samples.
  3. cAgreement of results between RT-LAMP and qRT-PCR, κ = 0.939.
  4. dOne of RT-LAMP positive samples were tested negative by qRT-PCR
  5. eSix of qRT-PCR positive samples were tested negative by RT-LAMP.
  6. fDENV RNA was tested negative by both RT-LAMP and qRT-PCR in 7 samples.