Figure 2From: Protective immunity against Toxoplasma gondiiinduced by DNA immunization with the gene encoding a novel vaccine candidate: calcium-dependent protein kinase 3 Identification of CDPK3 expression in E. coli BL21 (DE3) by SDS-PAGE and western-blotting. M: protein molecular weight marker; lane 1, recombinant pET-30a (+); lane 2, rTgCDPK3 induced for 8Â h with 0.6Â mmol/L IPTG; lane 3, Elution result of 20Â mmol/L imidazole solution; lane 4, Elution result of 50Â mmol/L imidazole solution; lane 5, Western blotting analysis with the sera against pVAX-CDPK3; lane 6, Western blotting analysis with the sera before immunization.Back to article page