Figure 3

Immunofluorescence staining of CCR7 in lung tissue. Staining shows co-localization of CCR7 with endothelial cells, fibroblasts and macrophages in murine lungs infected with R. conorii (lethal infection). Lung sections were stained with CCR7 in combination with endothelial, fibroblast and macrophage cell markers. CCR7 was counterstained with Alexa Fluor 488 conjugated goat anti-rabbit IgG (green). Endothelial and fibroblast cell markers were counterstained with Alexa Fluor 568 conjugated goat anti-rat IgG (red). Macrophages were counterstained with Warp red chromogen. The nuclei were stained with Dapi (blue). Overlays are shown in the left panels. For a better visualization of co-localization of CCR7 with cell markers, inserts are overlays of only green and red staining. Similar results were obtained in 3 separate experiments.