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Table 1 Studies with controls of Chlamydia pneumoniae DNA detection in peripheral blood mononuclear cells

From: Association of circulating Chlamydia pneumoniaeDNA with cardiovascular disease: a systematic review

Reference Country Cases Prevalence (%) Controls Prevalence (%) Blood sampling DNA Extraction PCRa target
Berger, 2000 [21] Germany Vascular surgery 12 of 60 (20.0) Not stated 7 of 51 (13.7) 4 mL EDTA blood Phenol-chloroform 16S rDNA
Boman, 1998 [22] Sweden Coronary Angiography 60 of 101 (59.4) Blood donors 24 of 52 (46.1) 10 mL EDTAb blood Phenol-chloroform Nested MOMPc
Da Costa, 2000 (abstract) Germany Coronary Angioplasty and antibiotics 14 of 233 (6.0) Blood donors 5 of 200 (2.5) Not stated QIAamp or phenol-chloroform Not stated
Freidank, 2000 [23] Germany Carotid atherosclerosis 10 of 60 (16.7) Medical students, patients 0 of 109 (0.0) 10 mL whole blood QIAamp Nested pstI cloned fragment
Kaul, 2000 [24] United States Coronary artery disease 13 of 28 (46.4) Blood donors 5 of 19 (26.3) 20–30 mL EDTA, CD3+ cell selection SDS-proteinase K Nested MOMP HSPd-60
Maraha, 2001 [25] Netherlands Aortic aneurysm 18 of 88 (20.5) Healthy controls 8 of 88 (9.1) EDTA blood QIAamp 16S rDNA
Sessa, 2000 [26] Italy Unstable angina, myocardial infarction 24 of 93 (25.8) Healthy controls 2 of 42 (4.8) EDTA blood Phenol-chloroform Hemi-nested pstI cloned fragment
Smieja, 2001 [27] Canada Coronary Angiography or angioplasty 22 of 187 (11.8) Normal angiogram 2 of 21 (9.5) 8 mL BD CPTc tube QIAamp Nested MOMP
Wong, 1999 [28] United Kingdom Coronary Angiography 79 of 913 (8.7) Normal angiogram 21 of 292 (7.2) 4–5 mL EDTA blood Phenol-chloroform Nested MOMP
  1. a PCR, polymerase chain reaction b EDTA, ethylene diamine tetra-acetic acid c MOMP, major outer membrane protein d HSP, heat shock protein e CPT, BD cell preparation tube (BD Vacutainer Systems, Franklin Lakes NJ, USA)