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Figure 2 | BMC Infectious Diseases

Figure 2

From: A short purification process for quantitative isolation of PrPScfrom naturally occurring and experimental transmissible spongiform encephalopathies

Figure 2

Comparison of signal detection after blending PrP Sc positive and negative homogenates in different ratios in A) sheep scrapie and B) BSE. In each case, western blot analysis is shown for 100% positive tissue (top panels); 50% positive tissue (middle panels); and 6.25% positive tissue (bottom panels). Antibodies used were 6H4 (1:5000) and alkaline phosphatase labeled rabbit anti-mouse (1:5000), both from Prionics. Lanes show: C – 10% negative brain homogenate, unprocessed and without proteinase K digestion; BM – molecular weight marker. Amounts listed above other lanes indicate the amount of positive tissue equivalents loaded. Staining seen in the negative sample was considered to be due to improper digestion of the tissue. Amount of proteinase K used was 100 μg per ml homogenate in each case. The negative control and all test samples were processed by the described salt precipitation protocol. The signal seen in lane C may be attributed to cellular prion protein, PrPC, present in normal brain tissue.

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