B. recurrentis A1
is plasmid encoded and duplicated in isolate A1. A: Plasmid (lane 1 to 4) and chromosome-rich (lane 5 to 8) DNA were digested with EcoRI (lane 1 and 5), HindIII (lane 2 and 6), XbaI (lane 3 and 7), and DraI (lane 4 and 8), transferred to a membrane and hybridised under high stringency with the 715 bp vlp1 probe. B: Plasmid DNA from isolate A1 (lane 1 to 3) and isolate A17 (lanes 4 to 6) and total DNA from isolate A17 (lane 7) were digested with HindIII (lane 1 and 4), EcoRI (lane 2, 5, and 7), XbaI (lane 3 and 6), and treated as described above. C: Intact plasmids from isolate A1 (lane 1) and isolate A17 (lane 2) were separated by field inversion gel electrophoresis (predefined program P1, Biorad) and treated as described above.