Skip to main content

Table 1 Bacterial strains and plasmids. Plasmid pAWS2 was provided by Rosalind Van Landingham, pBluescript-int was provided by Bonnie Plikaytis, and pUS267 was provided by Jeremy Dale

From: I-TRAP: A method to identify transcriptional regulator activated promoters

Strains

Relevant Characteristics

Source/Reference

Electromax DH5α

Cloning host

Invitrogen

M. smegmatis LR222

Cloning host

[20]

M. smegmatis::SEint.2

constitutively expresses M. tuberculosis sigE gene

This study

M. smegmatisΔsigE

sigE knockout mutant

This study

M. tuberculosis H37Rv TMC102

Virulent laboratory strain

[21]

Plasmids

  

pAWS2

Source of the lacZ gene

R. Van Landingham

pBluescript-int

Source of integrase gene, int

B. Plikaytis

pCL5

Backbone for pCL8

[9]

pCL8

Source of the rpsL promoter

This study

pCRScript SK (+)

Vector with multiple cloning site

Stratagene

pMV261

Source of the hsp60 promoter and OriM

[22]

pPICZalphaC

Source of bleomycin resistance gene, ble

Invitrogen

pPR27

Source of gentamicin resistance gene, aacC1

[23]

pSE.1

pUC19 with promoterless aph and xylE genes

This study

pSE.2

pSE.1 with the hyg gene

This study

pSE.3

pSE.2 with the OriM

This study

pSE.4

promoter-trap vector

This study

pSEint.1

Integrating plasmid containing the ble gene

This study

pSEint.2

Integrating plasmid, constitutive M. tuberculosis sigE expression

This study

pSEKO.1

5' region of the M. smegmatis sigE gene

This study

pSEKO.2

pSEKO.1 with 3' end of M. smegmatis sigE gene

This study

pSEKO.3

pSEKO.2 with the aacC1 gene

This study

pSEKO.4

Suicide plasmid with interrupted M. smegmatis sigE gene

This study

pT7Blue

Blunt cloning vector

This study

pTKMX97

Source of the xylE gene

[10]

pUC19

Vector with multiple cloning site

Amersham Biosciences

pUC4-KIXX

Source of aph gene

Amersham Biosciences

pUS267

Source of hygromycin resistance gene, hyg

J. Dale