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Table 2 Oligonucleotide primers. Underlined bases are restriction sites relevant to the construction. MS: Mycobacterium smegmatis; MTB: Mycobacterium tuberculosis; P hsp60: promoter for the M. tuberculosis hsp60 gene. PrpsL: promoter for the M. smegmatis rpsL gene

From: I-TRAP: A method to identify transcriptional regulator activated promoters

Primer Designation

Sequence 5' to 3'

Gene Amplified

Ble-N

ataaat ggatccaatggccaagttgaccagt

ble

Ble-C

aaataa ggatcctcagtcctgctcctcggc

ble

TN9-5R

cagggcaccggacaggtcggtc

aph

981816

atgtacgtggcgaactccgttgta

P hsp60

981819

gatgatatatttttatcttgtgca

P hsp60

987916

gggcccaa ggatccaatggaactcctcggcggaccc

MTBsigE

987917

agcgaactgggttgacgtgaactgc

MTBsigE

106777

tggaactcctcggcggacc

MTBsigE

106778

tgacgtgaactgcgcactcg

MTBsigE

7208

tgcga gttaaccgaaccccgagcagatctacgacg

MSsigE

7209

gcatctctagaagccgatcgcgtgtcggcgtc

MS sigE

7206

gcatcaagctttgctctagatcggagatcgaccgtttctg

MSsigE

7207

tcggt gttaaccctcggcgggcacgcggtcg

MSsigE

7204

cgtga gttaacctcgcgcagcgtgggtgc

aacC1

7205

gaccg gttaacggcgttgtgacaatttaccgaac

aacC1

7997

cctcgacatggtgcgccg

MSsigE

7998

caggcgcgaatcgtggtag

MSsigE

7999

gccgtctgcccaattgcag

MTBsigE

1915

gatca ggatccgatcaagagac

aph

1916

gcattacacttttgttcatttcgaaccccagagtcccgctc

aph

1917

agcgggactctggggttcgaaatgaacaaaggtgtaatgcg

xylE

1918

ccgggccggaccatcaggtcagc

xylE

5077

cgtgc tctagagcgagagtagggaactgc

rRNA

5078

CGTCGT CTAGAGACGACAGGAAGAGTTTGTAG

rRNA

47

CGGAGGAATCACTTCGCAATG

hsp60

39A

GGAGTTGAAGGCGATCTGCTT

hsp60

MA1

GTGCCCGCACGCCG GGTACCATGAACTATGAGG

PrpsL

MA2

CCTCATAGTTCAT GGTACCCGGCGTGCGGGCAC

PrpsL

TK97L

TCGA AAGCTTCGTCGCGGTCGGCCC

PrpsL