Comparison of two DNA targets for the diagnosis of Toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes

Table 1 Oligonucleotide primers and LightCycler hybridization probes used in the PCR assay.

Oligo-nucleotide	Sequence ^a	Target gene	Nucleotide position	GenBank accession no.	Reference
Tox-9	AGG AGA GAT ATC AGG ACT GTA G	Cryptic	143–164	AF487550	Present study
Tox-11	GCG TCG TCT CGT CTA GAT CG	Cryptic	304–285	AF487550	Present study
Tox-HP-1	GAG TCG GAG AGG GAG AAG ATG TT-[FL]	Cryptic	214–236	AF487550	Present study
Tox-HP-2	[Red 640]-CCG GCT TGG CTG CTT TTC CTG-Ph	Cryptic	238–258	AF487550	Present study

^a [FL], fluorescein; [Red 640], LightCycler-Red 640-N-hydroxy-succinimide ester; [Ph], 3'-phosphate.

ISSN: 1471-2334