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Figure 6 | BMC Infectious Diseases

Figure 6

From: A comparative ultrastructural and molecular biological study on Chlamydia psittaci infection in alpha-1 antitrypsin deficiency and non-alpha-1 antitrypsin deficiency emphysema versus lung tissue of patients with hamartochondroma

Figure 6

Detection of chlamydiae by nested omp A-PCR from frozen lung tissue of patients with advanced emphysema (samples N1 to N43). DNA was extracted from tissue samples, subjected to nested amplification, and PCR products were electrophoresed on 2% agarose gels. The amplicon of approximately 400 bp is specific for Chlamydia psittaci. Strain DC 5 of Chlamydophila psittaci was used as positive amplification control, nc1 and nc2 are negative (reagent) controls. Lane M shows the 100-bp ladder (Invitrogen, Karlsruhe, Germany).

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