Vaccine candidates derived from a novel infectious cDNA clone of an American genotype dengue virus type 2

BMC Infectious Diseases

Table 3 DEN-2 Tonga/74 is poorly infectious for Aedes aegypti fed an infectious bloodmeal, and the Δ30 mutation does not decrease infectivity for Toxorynchites amboinensis.

	A. aegypti (oral infection)				T. amboinensis (intrathoracic inoculation)
Virus	Dose (log₁₀PFU)^a	No. tested	% Infected^b		Dose (log₁₀PFU)^c	No. tested	% Infected^d
			Midgut	Head
DEN-2 Tonga/74	2.8	20	0	0	--	--	--
rDEN-2	3.5	18	0	0	2.9	5	100
					1.9	12	83
					0.9	8	63
					-0.1	7	14
					-1.1	7	0
							MID₅₀ = 0.9
rDEN2Δ30	3.5	22	0	0	2.7	8	100
					1.7	7	100
					0.7	6	83
					-0.3	7	43
					-1.3	4	50
					-2.3	5	0
							MID₅₀ = -0.2

^a Amount of virus ingested, assuming a 2 ul bloodmeal.
^b Percentage of mosquitoes with antigen detectable by IFA in midgut or head tissue prepared 21 days after oral infection.
^c Amount of virus present in 0.22 ul inoculum.
^d Percentage of mosquitoes with antigen detectable by IFA in head tissue prepared 14 days post-inoculation.

ISSN: 1471-2334