Description of material | Growth on TSA + 5% SBA @ 48 hrs | Growth on Middlebrook 7H11 | Kinyoun Stain direct from positive 12B | Probe | Conclusion |
---|---|---|---|---|---|
RFLP lysate – before CTAB addition (2 vials) | - | +, MTB morphology | AFB+ (4+), serpentine cording | ND | Viable MTB 1 |
RFLP lysate from frozen storage #1 | - | +, KS of growth AFB - | 4 AFB/slide, morph not consistent with TB | MTB- | contaminant present – ubiquitous mycobacteria |
RFLP lysate from frozen storage #2 | - | +, KS of growth AFB - | 15 AFB/slide, morph not consistent with TB | MTB- | contamination present – ubiquitous mycobacteria |
RFLP lysate from frozen storage #3 & #4 | - | +, KS of growth AFB - | No AFB | contamination present | |
Boiled lysate | - | +, Smooth colonies not consistent with MTB | AFB+ 5–10/field, clumping, no obvious serpentine cording | MTB- | ubiquitous mycobacteria |
Smear, 1 h at 95°C & Smear, 2 h at 95°C2 | - | +, MTB morphology | AFB+ (4+), serpentine cording | MTB+ | Viable MTB |
Protein extracts (2) | - | +, MTB morphology | AFB+ (4+), serpentine cording | MTB+ | Viable MTB |