Figure 3
The effect of extracellular pH on intracellular pH of PBMCs as a function of time. PBMCs labeled intracellularly with pH sensitive fluorescent indicators (Oregon Green or carboxyfluorescein), were incubated under varying acidic conditions and monitored over time. Upon acidification, PBMC intracellular pH undergoes a rapid decrease to or nearly to extracellular pH. At and above pHe 6.0, PBMCs recover their normal intracellular pH (7.3) after 5–10 minutes. At pHe 5.5 and below, PBMCs are unable to recover their intracellular pH. Points are mean (± SD) of three experiments.