Table 1 Primers and probes
From: A simple and rapid approach for screening of SARS-coronavirus genotypes: an evaluation study
Sequence (all sequence starts from the 5' end) | |
|---|---|
SNV 17564* | |
Forward primer | GACACTGTGAGTGCTTTAGTTTATGACA |
Reverse primer | CCTTTGTAGAACATTTTGAAGCATTG |
Probes | FAM-AGCTGACTTATCCTTGTGT |
VIC-AGCTGACTTCTCCTTGTGT | |
Synthetic template for allele T | GTTGACACTGTGAGTGCTTTAGTTTATGACAATAAGCTAAAAGCACACAAGGATAAGTCAGCTCAATGCTTCAAAATGTTCTACAAAGGTGT |
Synthetic template for allele G | GTTGACACTGTGAGTGCTTTAGTTTATGACAATAAGCTAAAAGCACACAAGGAGAAGTCAGCTCAATGCTTCAAAATGTTCTACAAAGGTGT |
SNV 21721 | |
Forward primer | CCATTTTATTCTAATGTTACAGGGTTTCA |
Reverse primer | TTTCTCTGTGGCAGCAAAATAAATAC |
Probes | FAM-ATACGTTTGGCAACCCTGTC |
VIC-ATACGTTTGACAACCCTGTC | |
Synthetic template for allele G | CTTCCATTTTATTCTAATGTTACAGGGTTTCATACTATTAATCATACGTTTGGCAACCCTGTCATACCTTTTAAGGATGGTATTTATTTTGCTGCCACAGAGAAATCA |
Synthetic template for allele A | CTTCCATTTTATTCTAATGTTACAGGGTTTCATACTATTAATCATACGTTTGACAACCCTGTCATACCTTTTAAGGATGGTATTTATTTTGCTGCCACAGAGAAATCA |
SNV 22222 | |
Forward primer | GAGCCATTCTTACAGCCTTTTTA |
Reverse primer | GCCAACAAAATAGGCTGCAG |
Probes | FAM-TGCTCAAGACACTTGGG-MGB |
VIC-TGCTCAAGACATTTGGG-MGB | |
Synthetic template for allele C | GCCATTCTTACAGCCTTTTTACCTGCTCAAGACACTTGGGGCACGTCAGCTGCAGCCTATTTTGTTGGCTATTTAAAGCCAACTACATTTATGCTCAAGTATGATG |
Synthetic template for allele T | GCCATTCTTACAGCCTTTTTACCTGCTCAAGACATTTGGGGCACGTCAGCTGCAGCCTATTTTGTTGGCTATTTAAAGCCAACTACATTTATGCTCAAGTATGATG |
SNV 23823 | |
Forward primer | TCGCTCAAGTCAAACAAATGTACA |
Reverse primer | GAGGGTCAGGTAATATTTGTGAAAAATT |
Probes | FAM-CCAACTTTGAAATATTTTGG |
VIC-CAACTTTGAAAGATTTTGG | |
Synthetic template for allele T | TGTTCGCTCAAGTCAAACAAATGTACAAAACCCCAACTTTGAAATATTTTGGTGGTTTTAATTTTTCACAAATATTACCTGACCCTCTAA |
Synthetic template for allele G | TGTTCGCTCAAGTCAAACAAATGTACAAAACCCCAACTTTGAAAGATTTTGGTGGTTTTAATTTTTCACAAATATTACCTGACCCTCTAA |
SNV 27827 | |
Forward primer | TCATTGTTTTGACTTGTATTTCTCTATGC |
Reverse primer | CTTCAAGCACATGAGGTTTATTAGATG |
Probes | FAM-TTGCATATGCACTGTAGT |
VIC-TTGCATACGCACTGTAGT | |
Synthetic template for allele C | TTCTCATTGTTTTGACTTGTATTTCTCTATGCAGTTGCATATGCACTGTAGTACAGCGCTGTGCATCTAATAAACCTCATGTGCTTGAAGATCC |
Synthetic template for allele T | TTCTCATTGTTTTGACTTGTATTTCTCTATGCAGTTGCATACGCACTGTAGTACAGCGCTGTGCATCTAATAAACCTCATGTGCTTGAAGATCC |