Figure 2

Chlamydiae within Peripheral Blood Transfer Infection when Cultured in vitro on J774A.1 host cell monolayers. BC from NBD peripheral blood samples were isolated, lysed and layered onto host cell monolayers as described. Epifluorescence images in Panel A show a representative example of a 96 h pi culture of a BC lysate from a Chlamydia smear positive sample and demonstrate these monolayers have remained healthy. Panel B shows a representative example of 96 h pi culture of a BC lysate from a Chlamydia negative smear sample. Note: panel A contains numerous cells with large inclusions, as well as cells with small inclusions and extracellular chlamydiae. These are characteristic of multiple replicative rounds in culture. Photographed using a Nikon Eclipse E600 epifluorescence microscope and a SPOT digital camera Original magnification 400×