Table 2 Comparative rate of apoptosis and biosynthetic activity of blood monocytes of four animal groups with acute pyelonephritis by multidrug-resistant Pseudomonas aeruginosa. Rate of apoptosis was expressed by the activity of cytoplasmic caspase-3; biosynthetic activity by the ex vivo release of tumor necrosis factor-alpha (TNFα). Therapy was administered 1.5 hours after bacterial challenge.
Time (hours) after bacterial challenge | A (controls) | B (clarithromycin) | C (amikacin) | D (clarithromycin + amikacin) |
|---|---|---|---|---|
Cytoplasmic activity of caspase-3 (Mean ± SE, pmol/min.104 cells) | ||||
3.5 | 1810.2 ± 1779.6 | 3210.3 ± 1173.5 | 277.5 ± 273.0 | 1155.7 ± 647.8 |
24 | 2187.0 ± 1205.9 | 1096.6 ± 431.9 | 663.7 ± 662.2 | 647.9 ± 453.1 |
48 | 1129.5 ± 863.3 | 908.1 ± 908.1 | 6575.5 ± 657.8 | 112.3 ± 80.6 |
Ex vivo release of TNFα (Mean ± SE, pg/10 4 cells) | ||||
3.5 | 96.6 ± 61.9 | 394.6 ± 315.5 | 132.2 ± 44.3 | 342.7 ± 259.9 |
24 | 60.5 ± 39.9 | 34.5 ± 15.8 | 143.3 ± 126.5 | 10.6 ± 3.9a |
48 | 63.1 ± 25.1 | 15.9 ± 10.6b | 79.4 ± 68.4 | 71.7 ± 19.1 |