Figure 3

Association of gp-340 I with increased S. mutans adhesion. (A-C) Bacterial adhesion to purified gp-340 I toIII proteins coated on hydroxyapatite surfaces (2 ug/ml for fixed amounts of protein). Data are presented as mean ± SE from double measurements of repeated experimental runs. (A) Adhesion of S. mutans Ingbritt to gp-340 I vs II or III (* p < 0.001, respectively). Similar results were obtained irrespective of culturing in BHI or Jordan broth. (B) Adhesion of S. mutans NG8 and isogenic mutant 834, disrupted in the pac (AgI/II polypeptide) gene, to gp-340 I protein (* p < 0.001). (C) Adhesion of L. lactis expressing SpaP (from S. mutans) or vector control (wild-type) L. lactis MG1363 to serial dilutions of gp-340 I vs II or III (* p < 0.001, respectively). Similar results were obtained when L. lactis expressing PAc was bound to serial dilutions of gp-340 I to III.