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Table 1 Primer sequence, primer concentration and predicted length of PCR products for multiplex PCR used to identify ETEC, EPEC, EHEC, EIEC and EAEC

From: Identification of diarrheagenic Escherichia coli isolated from infants and children in Dar es Salaam, Tanzania

Assay

Target gene

Oligonucleotide sequence (5' to 3')

Ampl Size (bp)

Reff

 

elt

F. CTCTATGTGCACACGGAGC

322

12

  

R. CCATACTGATTGCCGCAAT

  

Assay n1

St1a

F.TCTTTCCCCTCTTTTAGTCAGTC

170

11

  

R. CCGCACAGGCAGGATTAC

  
 

uidA

F. CCAAAAGCCAGACAGAGT

623

11

  

R. GCACAGCACATCAAAGAG

  
 

eae

F. TGATAAGCTGCAGTCGAATCC

229

11

Assay n2

 

R. CTGAACCAGATCGTAACGGC

  
 

bfpA

F. CACCGTTACCGCAGGTGTGA

450

11

  

R. GTTGCCGCTTCAGCAGGAGT

  
 

stx 1

F. GAAGAGTCCGTGGGATTACG

130

11

  

R. AGCGATGCAGCTATTAATAA

  

Assay n3

Stx 2

F.GGGTACTGTGTGCCTGTTACTGG

510

11

  

R. GCTCTGGATGCATCTCTGGT

  
 

ial

F.CTGGTAGGTATGGTGAGG

320

11

  

R. CCAGGCCAACAATTATTTCC

  
 

St1b

F.ATTTTTCTTTCTGTATTGTCTT

190

15

Assay n4

 

R.CACCCGGTACAAGCAGGATT

  
 

uidA

F. CCAAAAGCCAGACAGAGT

623

11

  

R. GCACAGCACATCAAAGAG

  
 

aggR

F. CTAATTGTACAATCGATGTA

457

12

Assay n5

 

R. AGAGTCCATCTCTTTGATAAG

  
 

Aat

F. CTGGCGAAAGACTGTATCAT

629

12

  

R. CAATGTATAGAAATCCGCTGTT

  
  1. F. forward primers, R. reverse primers.