Figure 5

NOD2 mRNA expression in live mycobacteria stimulated-peripheral blood cells obtained from healthy volunteers. NOD2 mRNA levels varied widely in healthy donor derived-PBMCs that were stimulated with live mycobacterial strains [A = combined data and B to F individual data]. NOD2 mRNA expression was analysed, by real-time RT-PCR, in peripheral blood cells isolated from healthy donors. To permit comparison between individuals, absolute copy numbers of NOD2 mRNA were measured during RT-PCR, using cDNA standards, and expressed relative to 10⁶ mRNA copies of a validated housekeeping gene HuPO. Lower numbers of PBMCs were harvested from Donors 1 and 3, and these cells were not stimulated with Mycobacterium vaccae. MV = M, vaccae; H37 = H37RV strain and CPA = Beijing strain.