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Table 3 Confirmation of B. cenocepacia gene expression by Real Time PCR

From: Gene expression changes linked to antimicrobial resistance, oxidative stress, iron depletion and retained motility are observed when Burkholderia cenocepaciagrows in cystic fibrosis sputum

 

Real-time PCR fold change using delta delta CT method

Gene expression determined from

(infection; clinical condition):

BCAL0270

(ferric reductase

transmembrane component)

BCAM2753

(ohrgene, organic

hydroperoxide resistance protein)

BCAL1107

(oxidoreductase)

BCAM1676

(nitrite/sulfite

reductase)

Microarray dataset

(Sputum 1, 2, and 3; see below)

↑ 36

↑ 8

↑ 66

↑ 20

Sputum 2

(B. cenocepacia; stable)

↑ 160

↑ 100

↑ 914

↑ 97

Sputum 3

(B. cenocepacia; exacerbation)

↑ 65

↑ 40

↑ 299

↑ 4

Sputum 4

(B. cenocepacia; stable)

↑ 25

↑ 22

↑ 235

-

Sputum 5

(P. aeruginosa; exacerbation)

↑ 22

↑ 71

↑ 189

-

Sputum 6

(P. aeruginosa; stable)

↑ 3

↑ 3

↑ 12

↑ 94

Average for sputum growth

↑ 55

↑ 47

↑ 330

↑ 65

Fold change in minimal media with:

    

No FeSO 4

↑ 2

-

  

0.1% H 2 O 2

-

↑ 5

  

0.15% H 2 O 2

-

↑ 3

  

0.002% t-butyl hydroperoxide

-

↑ 2

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