Figure 4

Pseudo color cluster view of up regulated (a) and down regulated genes (b) in PBMC in response to 2 μg/ml the rPA ₂₀ . Cells were obtained from 4 different donors and were treated with the rPA₂₀. RNA was isolated and hybridized on the cDNA microarray slides as detailed in Materials and Methods. Images were analyzed using GenePix 4.0 and data were analyzed using GeneSpring 7.0. The expression data for these genes are listed in table 1. These genes were identified, using ANOVA t-test with a p < 0.05, to be highly significantly regulated when compared to the control untreated cells.