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Figure 5 | BMC Infectious Diseases

Figure 5

From: Activity of the Bacillus anthracis20 kDa protective antigen component

Figure 5

A. Cluster view of gene expression profiles in PBMC exposed to 2 μg/ml native and denatured rPA 20 compared to the control untreated. The peptide was heat denatured at 95°C for 15 min. prior to adding it to the cells. Equal amounts of the native and denatured peptides were added to the cells and incubated for 4 hrs. Data were normalized to the control untreated cells. B. Expression levels of TNF-α in PBMC cells exposed to the native and denatured rPA20. Cells were incubated with 2 μg/ml of the two peptides separately and the expression of TNF-α was examined using ELISA.

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