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Table 2 Coefficient of variation (CV%) for the One Step real time RT-PCR assay.

From: Development and evaluation of a real-time one step Reverse-Transcriptase PCR for quantitation of Chandipura Virus

(Copies of IVT RNA)

Ct

SD

CV%

1010

11.25

0.34

3.02

109

14.71

0.87

5.91

108

19.71

0.30

1.52

107

23.14

0.54

2.33

106

26.88

0.45

1.67

105

30.39

0.46

1.51

104

33.81

0.40

1.18

103

35.80

0.56

1.56

102

38.91

0.53

1.36

  1. Standard Deviation (SD) and mean Ct vales were used for the calculation of CV%. Minimum and maximum vales as have shown in bold.
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BMC Infectious Diseases

ISSN: 1471-2334

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