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Table 4 Dot- blot ELISA of immunotypes using Monoclonal antibodies for N. meningitidis.

From: Storage and stability of IgG and IgM monoclonal antibodies dried on filter paper and utility in Neisseria meningitidis serotyping by Dot-blot ELISA

Specificity

Mabs

  

1 week

4 weeks

1 year

13 years

4 weeks

RT

   

isotypes

dilution(a)

dilution(b)

dilution(a)

dilution(b)

dilution(a)

dilution(b)

dilution(b)

dilution(a)

dilution(b)

L3,7,9

9-2-L379

WRAIR 4BE12C10

IgG2a

1:100,000

1:100,000

1:100,000

1:100,000

1:100,000

1:100,000

1:10,000

1:100,000

1:100,000

L8

6E7-10

WRAIR6E710

IgM

1:100,000

1:100,000

1:100,000

1:100,000

1:100,000

1:100,000

1:10,000

1:100,000

1:100,000

L8

1C31B8

IAL

IgM

1.50,000

1.50,000

1.50,000

1.50,000

1.50,000

1.50,000

1:10,000

1.50,000

1.50,000

L1

1B81C3

IAL

IgM

1:10,000

1:10,000

1:10,000

1:10,000

1:10,000

1:10,000

1:10,000

1:10,000

1:10,000

L1

3G3-1-8C

WRAIR17-1-L1

n/d

1.50,000

1.50,000

1.50,000

1:10,000

1:10,000

1:10,000

1:10,000

1:10,000

1:10,000

  1. WRAIR = Walter Reed Army Institute of Research (USA).
  2. NVI = Netherlands Vaccine Institute (previously the RIVM).
  3. Adolfo Lutz Institute (IAL). The Mabs were prepared in Immunology Section by EN De Gaspari.
  4. RT = Room Temperature (30 days).
  5. dilution(a) (Mab) freezer -20°C.
  6. dilution(b) (Mab) eluted of dried filter paper.
  7. Mabs = monoclonal antibodies