Figure 2

PMN antimicrobial functions from control (non-infected) and septic rats. Naive, non-infected control rats, naive rats treated with G-CSF only (20 μg/kg, 12 h before and 12 h after PCI), PCI rats without prophylaxis, PCI rats with antibiotic prophylaxis (coamoxiclav = CoA or ceftriaxone/metronidazole = Cef/met) and PCI rats with antibiotic plus G-CSF prophylaxis were analysed. Blood was sampled at 24 h after PCI from n = 9 animals each condition. Migration was assessed with a Boyden camber test. Granulocyte superoxid anion formation was determined with a cytochrom-C microplate assay. Data are presented as mean ± SEM. ANOVA analysis of variance revealed for migration P < 0.05 and for superoxide anion formation P < 0.001, df = 6 and post hoc tests of with Bonferroni correction obtained *P < 0.01 versus control, **P < 0.001 versus control, ^#P < 0.05 versus PCI, ^##P < 0.01 versus PCI, ^§P < 0.05 versus coamoxiclav.