Brain injury-associated biomarkers of TGF-beta1, S100B, GFAP, NF-L, tTG, AbetaPP, and tau were concomitantly enhanced and the UPS was impaired during acute brain injury caused by Toxocara canisin mice

Table 1 Primers of biomarkers, and their source, PCR conditions, and expected size.

Targets	Primer sequence 5'-3'	Annealing temperature	Cycle number	Expected size (bp)	Reference
TGF-β1	sens-AGACGGAATACAGGGCTT TCGATT CA	55°C	35	492	[73]
	antisens-CTTGGGCTTGCGACCCAGTAGTA
GFAP	sens-GAATGGCCACTAAGGCAGTC	58°C	35	400	[74]
	antisens-TGCACTCCCTCTCTCCTGTT
S100B	sens-GACTCCAGCAGCAAAGGTGAC	58°C	35	211	[75]
	antisens-CATCTTCGTCCAGCGTCTCCA
tTG	sens-AGGCCAACCACCTGAACAAA	60°C	35	475	[76]
	antisens-CATACAGGGGATCGGAAAGT
NF-L	sens-AGCAGAATGCAGACATTAGCG CC	57°C	38	200	[77]
	antisens-TGGTCTCTTCGCCTTCC AAGA GT
AβPP69	sens-GCACTAACTTGCACGACTAT GGCATGCTGCTGCCCTG	70°C	35	401
	antisens-GCTGGCTGCCGTCGTGG GAACTCGGACTACCTCCTCCAC A
AβPP75 1	sens-CTACCACTGAGTCTGTGGAG	64°C	45	222	[50]
	antisens-GCTGGCTGCCGTCGTGG GAAACACGCTGCCACACACCGC C
AβPP77 0	sens-CTACCACTGAGTCTGTGGAG	64°C	45	242
	antisens-CTTGAGTAAACTTTGGGA TGACACGCTGCCACACACCGCC
GAPDH	sens-ACCACAGTCCATGCCATCAC	60°C	27	452	[75]
	antisens-TCCACCACCCTGTTGCTG TA

ISSN: 1471-2334