Truncation in the tcdC region of the Clostridium difficilePathLoc of clinical isolates does not predict increased biological activity of Toxin B or Toxin A

Table 1 Primers used in this evaluation.

Sequence	primer	Gene	Reference
AAAGAAGCTACTAAGGGTACAAA	tpi-F	tpi	[26]
CATAATATTGGGTCTATTCCTAC	tpi-R		[26]
AGATTCCTATATTTACATGACAATAT	tcdA-F	tcdA	[26]
GTATCAGGCATAAAGTAATATACTTT	tcdA-R		[26]
AATGCATTTTTGATAAACACATTG	tcdB-3	tcdB	this study
AAGTTTCTAACATCATTTCCAC	tcdB-4		this study
TCTCTACAGCTATCCCTGGT	PaL15	tcdC	[12]
AAAAATGAGGGTAACGAATTT	Pal16		[12]
TTTCATACATTTGTGCTGGG	cdd1-A	tcdC	this study
AATGCATTTTTGATAAACACATTG	tcdC-3		this study
TTCTAGATTTCATAAAAGATAC	TPR-1	tcdR	this study
CTGACATATTATGATATTCTTC	tcdB-UP		this study
GTTGTTTAGATTTAGATGAAAAGA	Lok6	tcdE	[33]
CTTGGTCTAATGCTATATGCGAG	PrimexA		[34]
CTTAATGCAAGTAAATACTGAG	cdtB-pos	cdtB	[24]
AACGGATCTCTTGCTTCAGTC	cdtB-rev		[24]

ISSN: 1471-2334