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Table 3 Survival of organisms embedded in TBF or BBF1 following HLD2: indirect qualitative outgrowth testing compared to quantitative viability3 results

From: Modeling microbial survival in buildup biofilm for complex medical devices

Microorganism:

BFF

Average cfu/peg4 (Log10)

Outgrowth5

# Positive/#Tested

Average time to detection (days) for outgrowth

HLD: 2.6% GLUT:

   

P. aeruginosa:

   

TBF – Day 3

<LD6

3/9

4

TBF – Day 15

3.7

7/9

1

TBF – Day 30

4.8

9/9

1

BBF – Day 3

<LD

4/9

20

BBF – Day 15

<LD

2/9

10

BBF – Day 30

1.9

2/9

1

HLD: 7% AHP:

   

P. aeruginosa:

   

TBF – Day 3

<LD

0/9

-

TBF – Day 15

1.6

4/9

1

TBF – Day 30

2.5

4/9

1

BBF – Day 3

<LD

0/9

-

BBF – Day 15

<LD

0/9

-

BBF – Day 30

<LD

0/9

-

  1. 1 3-day cyclical drying/HLD in ATS medium
  2. 2 HLD for cyclic BBF pegs and sample pegs of TBF (formed in ATS)
  3. 3 Quantitative viability testing on replicate pegs (without enhanced recovery protocol)
  4. 4 Average of 9 pins
  5. 5 Indirect qualitative outgrowth (enhanced recovery protocol):
  6. TBF or BBF peg placed in 10%FBS-TSB, shake/sonicate/vortex (as per extraction
  7. method), then continue to incubate at 35°C/30d
  8. 6LD: limit of detection is 10 cfu/peg for quantitative viability counts