Skip to main content
Figure 2 | BMC Infectious Diseases

Figure 2

From: Cytometric detection of antigen-specific IFN-γ/IL-2 secreting cells in the diagnosis of tuberculosis

Figure 2

Analysis of antigen-specific cytokines secreted by T cells. A) Frequency of cytokine secreting cells after 18 (left panel) or 72 (right panel) hours of culture with the positive control SEA, PPD, ESAT-6/CFP-10 or BCG in PBMC of controls (CTR, black bars), patients with active TB (TB Act, white bars) or individuals with latent TB infection (TB Lat, grey bars). Columns represent median values ± standard error of the frequency (%) of the total cytokines (IFN-γ, IL-2 and IFN-γ/IL-2) secreting cells calculated on CD16- total T cells. Only the statistically significant (p-values < 0.05) differences among the indicated populations were reported. * indicates p < 0.05; *** indicates p < 0.005. B) Frequency of cytokine secreting cells among the CD8+ (left panel) or the CD4+ (right panel) cells in PBMC of controls (CTR), patients with active TB (TB Act) or individuals with latent TB infection (TB Lat) after 72 hours of culture with PPD, ESAT-6/CFP-10 or BCG. Columns represent median values ± standard error. Differences among the indicated populations were considered significant when the p-values were < 0.05. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.005; n.s. = not significant.

Back to article page