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Figure 2 | BMC Infectious Diseases

Figure 2

From: Overexpression of activated protein C hampers bacterial dissemination during pneumococcal pneumonia

Figure 2

APC-overexpression does not impact on lung pathology but attenuates neutrophil influx into the lungs . Histology scores were similar in WT and APChigh-mice 6, 24 and 48 hours post-infection with 5*104 CFU of S. pneumoniae (A). Representative photographs of WT (B) and APChigh-mice (C) (H&E staining, original magnification x100) 48 hours post-infection. Decreased granulocyte influx as measured by Ly-6G expression in APChigh-mice infected with S. pneumoniae, 24 hours after infection (D). Representative photographs of Ly-6G immunostaining (original magnification x100) for granulocytes of WT (E) and APChigh-mice (F), 24 hours post-infection. APChigh-mice showed decreased numbers of total cell counts in bronchoalveolar lavage fluid (BALF) compared to WT mice (G), which was caused by a decreased influx of neutrophils (H). No differences in influx of macrophages (I) and lymphocytes (J) were found. Data are expressed as box and whisker plots showing the smallest observation, lower quartile, median, upper quartile and largest observation. Numbers of Ly-6G + granulocytes are expressed as the percentage of the total lung surface area. Neutrophils, macrophages and lymphocytes are expressed as absolute numbers. Grey boxes represent WT mice, white boxes represent APChigh-mice (n =8 mice per group for each time point). *P <0.05 and **P <0.01 for WT versus APChigh-mice (Mann–Whitney U test).

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