Primary cultivation: factors affecting contamination and Mycobacterium ulcerans growth after long turnover time of clinical specimens

Table 3 Association ^§ between M. ulcerans growth and all factors of interest with a relevant effect

Factor	Value of factor	Odds ratio ^§	95% CI odds ratio	Overall effect p-value
Transport medium	7H9	0.304	0.046 - 2.026	<0.001
	dry	0.023	0.004 - 0.143
	Amies (ref. level)	1.000
Decontamination medium	OA_1h	0.414	0.102 - 1.683	0.519
	OA_30min	0.697	0.127 - 3.837
	NaOH_20min	1.242	0.204 - 7.546
	NaOH_10min (ref. level)	1.000
Inoculation media	LJ	0.478	0.148 - 1.545	0.216
Inoculation media	LJ_PANTA (ref. level)	1.000		0.216
Swab qPCR Ct value	for an increase in 1 unit Ct	0.879	0.775 - 0.996	0.044
Time from sampling to inoculation	for an increase in 10 days			0.001
Interaction of transport medium and days from sampling to inoculation	when 7H9^§§	0.531	0.320 - 0.881	0.074
	when dry^§§	0.880	0.704 - 1.099
	when Amies^§§ (ref. level)	0.541	0.347 - 0.844

^§Adjusted for random effects of the patient and swab.
^§§For an increase in 10 days from mean number of days from sampling to inoculation.

ISSN: 1471-2334