Nationwide high prevalence of CTX-M and an increase of CTX-M-55 in Escherichia coli isolated from patients with community-onset infections in Chinese county hospitals

Zhang, Jing; Zheng, Beiwen; Zhao, Lina; Wei, Zeqing; Ji, Jinru; Li, Lanjuan; Xiao, Yonghong

doi:10.1186/s12879-014-0659-0

Table 1 Primers used for PCR amplification of bla genes

From: Nationwide high prevalence of CTX-M and an increase of CTX-M-55 in Escherichia coli isolated from patients with community-onset infections in Chinese county hospitals

PCR target	Primer	Sequence (5′–3′)	Fragment size, bp	Ref
bla _TEM	TEM-F	CATTTCCGTGTCGCCCTTATTC	800	[27]
bla _TEM	TEM-R	CGTTCATCCATAGTTGCCTGAC	800	[27]
bla _SHV	SHV-F	GGTTATGCGTTATATTCGCC	867	[28]
bla _SHV	SHV-R	TTAGCGTTGCCAGTGCTC	867	[28]
CTX-M-1 group	CTX-M-1F	CCCATGGTTAAAAAATCACTGC	942	[7]
CTX-M-1 group	CTX-M-1R	CAGCGCTTTTGCCGTCTAAG	942	[7]
CTX-M-2 group	CTX-M-2F	CGACGCTAC CCCTGC TAT T	552	[29]
CTX-M-2 group	CTX-M-2R	CCAGCGTCAGATTTTTCAGG	552	[29]
CTX-M-8 group	CTX-M-8F	AACR(A)CR(G)CAGACGCTCTAC	326	[27]
CTX-M-8 group	CTX-M-8R	TCGAGCCGGAAGGTGTCAT	326	[27]
CTX-M-9 group	CTX-M-9F	ATGGTGACAAAGAGAGTGCAAC	876	This study
CTX-M-9 group	CTX-M-9R	TTACAGCCCTTCGGCGATGATT	876	This study
bla _OXA-1	OXA-1F	GGCACCAGATTCAACTTTCAAG	814	[27]
bla _OXA-1	OXA-1R	GACCCCAAGTTTCCTGTAAGTG	814	[27]
bla _OXA-2	OXA-2F	GGCACCAGATTCAACTTTCAAG	702	[27]
bla _OXA-2	OXA-2R	GATTTGCTCCGTGGCCGAAA	702	[27]
bla _OXA-10	OXA-10F	ACGGCATTAGCTGGTTCAAT	720	This study
bla _OXA-10	OXA-10R	TGATTTTGGTGGGAATGGAT	720	This study
bla _GES	GES-F	AGTCGGCTAGACCGGAAAG	399	[27]
bla _GES	GES-R	TTTGTCCGTGCTCAGGAT	399	[27]
bla _PER	PER-F	GCTCCGATAATGAAAGCGT	520	[27]
bla _PER	PER-R	TTCGGCTTGACTCGGCTGA	520	[27]
bla _VEB	VEB-F	CATTTCCCGATGCAAAGCGT	648	[27]
bla _VEB	VEB-R	CGAAGTTTCTTTGGACTCTG	648	[27]
bla _CMY2	CMY2-F	AACACACTGATTGCGTCTGA	1228	[30]
bla _CMY2	CMY2-R	TCCTGGGCCTCATCGTCAGTTAT	1228	[30]
bla _CMY1	CMY1-F	TATTAGAGCGGTTTAGGCTG	1456	[30]
bla _CMY1	CMY1-R	AATGTACCGCCCTCTTTC	1456	[30]
bla _DHA	DHA-F	TGATGGCACAGCAGGATATTC	997	[27]
bla _DHA	DHA-R	GCTTTGACTCTTTCGGTATTCG	997	[27]
bla _KPC	KPC-F	CATTCAAGGGCTTTCTTGCTGC	538	[27]
bla _KPC	KPC-R	ACGACGGCATAGTCATTTGC	538	[27]

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ISSN: 1471-2334

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